Mar 5, 2021 · This SARS-CoV-2 whole-genome sequencing workflow is based on the 400 bp PCR tiling protocol and analysis methods devised by the ARTIC ...
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Here we evaluated PCR-tiling of short (~400-bp) and long (~2 and ~2.5-kb) amplicons combined with nanopore sequencing on a MinION device for analysis of the ...
The NextGenPCR is ideal to perform tiled PCR, due to its 3 separate temperature zones, which can be customised in temperature and heating time. A pre-mixed ...
Oct 26, 2021 · Multiplex tiling PCR is a desirable approach for targeted genome sequencing of SARS-CoV-2 in wastewater due to its low cost and rapid turnaround ...
Multiplex tiling PCR is a desirable approach for targeted genome sequencing of SARS-CoV-2 in wastewater due to its low cost and rapid turnaround time. However, ...
... This method involves random priming and avoids the selection of specific RNA sequences, thus minimizing the biases associated with amplicon-based sequencing ...
Primer design, coverage and pooling guide for BVDV-1 and BVDV-2 strains. Pooling was performed in accordance with the numbers of the tiles (1-4), resulting in ...
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This protocol uses extracted RNA samples; Includes reverse transcription and tiled PCR amplification; For multiplexing 1-96 samples; Library preparation ...
Oct 29, 2021 · Here we evaluated PCR-tiling of short (~400-bp) and long (~2 and ~2.5-kb) amplicons combined with nanopore sequencing on a MinION device for ...
Feb 12, 2020 · A quick guide to tiling ... tiling scheme. It is unlikely that assembly ... Aside from RNA fragment quality, PCR is less likely to work at very high ...