The NextGenPCR is ideal to perform tiled PCR, due to its 3 separate temperature zones, which can be customised in temperature and heating time. A pre-mixed ...
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Oct 26, 2021 · Multiplex tiling PCR is a desirable approach for targeted genome sequencing of SARS-CoV-2 in wastewater due to its low cost and rapid turnaround ...
Here we evaluated PCR-tiling of short (~400-bp) and long (~2 and ~2.5-kb) amplicons combined with nanopore sequencing on a MinION device for analysis of the ...
This protocol uses extracted RNA samples; Includes reverse transcription and tiled PCR amplification; For multiplexing 1-96 samples; Library preparation ...
Primer design, coverage and pooling guide for BVDV-1 and BVDV-2 strains. Pooling was performed in accordance with the numbers of the tiles (1-4), resulting in ...
Oct 29, 2021 · Here we evaluated PCR-tiling of short (~400-bp) and long (~2 and ~2.5-kb) amplicons combined with nanopore sequencing on a MinION device for ...
Multiplex tiling PCR is a desirable approach for targeted genome sequencing of SARS-CoV-2 in wastewater due to its low cost and rapid turnaround time. However, ...
PCR tiling of SARS-CoV-2 virus. This SARS-CoV-2 whole-genome sequencing workflow is based on the 400 bp PCR tiling protocol and analysis methods devised by ...